{"id":681,"date":"2023-03-15T14:35:54","date_gmt":"2023-03-15T06:35:54","guid":{"rendered":"https:\/\/www.cellnuo.com\/?page_id=681"},"modified":"2023-07-25T16:16:19","modified_gmt":"2023-07-25T08:16:19","slug":"%e9%87%8d%e7%bb%84%e6%a0%b8%e9%85%b8%e9%85%b6","status":"publish","type":"page","link":"https:\/\/www.cellnuo.com\/?page_id=681","title":{"rendered":"\u91cd\u7ec4\u6838\u9178\u9176"},"content":{"rendered":"\n

\u6765\u6e90 \uff1a<\/strong> \u91cd\u7ec4\u6838\u9178\u9176\uff0c\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002<\/p>\n\n\n\n

\u4e00. \u4ea7\u54c1\u7b80\u4ecb<\/strong><\/strong><\/p>\n\n\n\n

BenzoNuclease \u662f\u4e00\u79cd\u6765\u81ea\u4e8e\u7c98\u8d28\u6c99\u96f7\u6c0f\u83cc\uff08Serratia marcescens\uff09\uff0c\u7ecf\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u7684\u6838\u9178\u5185\u5207\u9176\u3002BenzoNuclease\u53ef\u964d\u89e3\u53cc\u94fe\u3001\u5355\u94fe\u3001\u7ebf\u72b6\u3001\u73af\u72b6\u7684DNA\u548cRNA\uff0c\u5b8c\u5168\u5c06\u6838\u9178\u964d\u89e3\u62103~5\u4e2a\u78b1\u57fa\u957f\u5ea6\u76845′-\u5355\u78f7\u9178\u5be1\u6838\u82f7\u9178\u3002\u56e0\u5176\u80fd\u9ad8\u6548\u964d\u89e3\u4efb\u4f55\u5f62\u5f0f\u7684 DNA \u548c RNA\uff0c\u53c8\u88ab\u79f0\u4e3a\u201c\u5168\u80fd\u6838\u9178\u9176\u201d\u3002 <\/p>\n\n\n\n

\u65e0\u8bba\u5728\u5b9e\u9a8c\u5ba4\u7814\u7a76\u8fd8\u662f\u5728\u5de5\u4e1a\u7ea7\u751f\u4ea7\u7684\u8fc7\u7a0b\u4e2d\uff0cBenzoNuclease\u662f\u53bb\u9664\u751f\u7269\u5236\u54c1\u4e2d\u6240\u6709\u5f62\u5f0fDNA\u548cRNA\u7684\u4e00\u79cd\u6709\u6548\u5de5\u5177\u9176\u3002\u901a\u8fc7\u9ad8\u6548\u7684\u6838\u9178\u53bb\u9664\uff0c\u53ef\u663e\u8457\u63d0\u5347\u540e\u7eed\u5b9e\u9a8c\u3001\u751f\u4ea7\u7684\u6548\u679c\u53ca\u4ea7\u91cf\uff0c\u6027\u80fd\u4f18\u4e8e\u5176\u4ed6\u6838\u9178\u53bb\u9664\u65b9\u6cd5\u3002<\/p>\n\n\n\n

\u4e8c. \u4ea7\u54c1\u7279\u6027<\/strong><\/strong><\/p>\n\n\n\n

\u9879\u76ee<\/strong><\/strong><\/td>\u6807\u51c6<\/strong><\/strong><\/td>\u65b9\u6cd5<\/strong><\/strong><\/td><\/tr>
\u5916\u89c2<\/td>\u65e0\u8272\u901a\u660e\u6eb6\u6db2<\/td>\u76ee\u6d4b\u68c0\u6d4b<\/td><\/tr>
\u6d3b\u6027<\/td>\u2265250KU\/ml<\/td>\u964d\u89e3\u9c91\u9c7c\u7cbe\u5b50DNA\u6cd5<\/td><\/tr>
\u7eaf\u5ea6<\/td>\u226599%<\/td>SDS-page\u7535\u6cf3\u68c0\u6d4b<\/td><\/tr>
\u7ec6\u83cc\u5185\u6bd2\u7d20<\/td>\u22640.25EU\/KU<\/td>\u836f\u5178\u68c0\u6d4b\u65b9\u6cd5<\/td><\/tr>
\u5bbf\u4e3bDNA<\/td>\u226410ppm<\/td>\u8367\u5149PCR\u65b9\u6cd5<\/td><\/tr>
\u5bbf\u4e3b\u6b8b\u4f59\u86cb\u767d(HCP)<\/td>\u226450ppm<\/td>\u836f\u5178\u68c0\u6d4b\u65b9\u6cd5<\/td><\/tr>
\u5fae\u751f\u7269\u9650\u5ea6<\/td>\u7b26\u5408\u89c4\u5b9a<\/td>\u836f\u5178\u68c0\u6d4b\u65b9\u6cd5<\/td><\/tr>
\u5206\u5b50\u91cf<\/td>~28KD<\/td>SDS-page\u7535\u6cf3\u68c0\u6d4b<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u6d3b\u6027<\/strong>\u5b9a\u4e49\uff1a<\/strong>\u572837\u2103\uff0cpH 8.0\u53cd\u5e94\u6761\u4ef6\u4e0b\uff0c2.625 mL\u53cd\u5e94\u4f53\u7cfb\u4e2d\uff0c\u572830 min\u5185\u4f7f\u25b3A260<\/sub>\u5438\u6536\u503c\u53d8\u53161.0\uff08\u76f8\u5f53\u4e8e\u5b8c\u5168\u6d88\u531637 \u03bcg\u9c91\u9c7c\u7cbeDNA\u6210\u4e3a\u5be1\u6838\u82f7\u9178\uff09\u6240\u7528\u7684\u9176\u91cf\u5b9a\u4e49\u4e3a\u4e00\u4e2a\u6d3b\u6027\u5355\u4f4d\uff08U\uff09\u3002<\/strong><\/p>\n\n\n\n

\u4e09. \u4f7f\u7528\u65b9\u6cd5<\/strong><\/strong><\/p>\n\n\n\n

1.\u7ec6\u80de\u5904\u7406\uff1aa.\u8d34\u58c1\u7ec6\u80de\u53bb\u9664\u57f9\u517b\u57fa\uff0c\u7528 PBS \u6d17\u540e\uff0c1mLRIPA \u88c2\u89e3\u6db2\uff08\u6216\u5176\u4ed6\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u88c2\u89e3\u6db2\uff09\u52a01-2uL\uff080.1-1\u03bcl\uff09\u6838\u9178\u9176\uff0c\u5ba4\u6e29\u6216\u51b0\u4e0a\u5b75\u80b2 5-30min\uff0c\u6536\u96c6\u88c2\u89e3\u6db2\uff0c\u79bb\u5fc3\u53d6\u4e0a\u6e05\u5373\u53ef\u8fdb\u884c\u4e0b\u6e38\u5b9e\u9a8c\u3002b.\u60ac\u6d6e\u7ec6\u80de\u79bb\u5fc3\u6536\u96c6\u540e\uff0c\u5728\u79bb\u5fc3\u7ba1\u4e2d\u52a0 1mLRIPA \u88c2\u89e3\u6db2\uff08\u6216\u5176\u4ed6\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u88c2\u89e3\u6db2\uff09\u52a01-2uL\uff080.1-1\u03bcl \uff09\u6838\u9178\u9176\uff0c\u5ba4\u6e29\u6216\u51b0\u4e0a\u5b75\u80b2 5-30min\uff0c\u6536\u96c6\u88c2\u89e3\u6db2\uff0c\u79bb\u5fc3\u53d6\u4e0a\u6e05\u5373\u53ef\u8fdb\u884c\u4e0b\u6e38\u5b9e\u9a8c\u3002<\/p>\n\n\n\n

2.\u7ec4\u7ec7\u6837\u54c1\uff1a\u5c06 30-100mg \u52a8\u7269\u6216\u8005\u690d\u7269\u7ec4\u7ec7\u7814\u78e8\u5145\u5206\u540e\uff0c\u52a0\u5165 100-200uL \u88c2\u89e3\u6db2\uff0c\u540c\u65f6\u52a0\u5165 0.5-1uL \u6838\u9178\u9176\uff0c\u5ba4\u6e29\u6216\u51b0\u4e0a\u5b75\u80b2 5-30min\uff0c\u6536\u96c6\u88c2\u89e3\u6db2\uff0c\u79bb\u5fc3\u53d6\u4e0a\u6e05\u5373\u53ef\u8fdb\u884c\u4e0b\u6e38\u5b9e\u9a8c\u3002<\/p>\n\n\n\n

3.\u5927\u80a0\u6746\u83cc\u6216\u8005\u5176\u4ed6\u7ec6\u83cc\uff1a\u7ec6\u83cc\u79bb\u5fc3\u6536\u96c6\u540e\uff0c\u7528\u88c2\u89e3\u6db2\u88c2\u89e3\u6216\u8005\u7814\u78e8\u7834\u788e\u540e\uff0c\u6bcf 1mL \u52a0 0.5-1uL \u6838\u9178\u9176\uff0c\u5ba4\u6e29\u5b75\u80b2 5-30min\uff0c\u6536\u96c6\u88c2\u89e3\u6db2\uff0c\u79bb\u5fc3\u53d6\u4e0a\u6e05\u5373\u53ef\u8fdb\u884c\u4e0b\u6e38\u5b9e\u9a8c\u3002<\/p>\n\n\n\n

\u6ce8\uff1a\u82e5\u6eb6\u6db2\u4e3a\u9ad8\u76d0\uff08>0.3MNaClass\uff09\u6eb6\u6db2\uff0c\u504f\u9178\u6027\u6216\u504f\u78b1\u6027\uff0c\u542b\u6709\u8f83\u9ad8\u6d53\u5ea6\u7684\u53bb\u57a2\u5242\u3001\u53d8\u6027\u5242\uff0c\u5e94\u9002\u5f53\u589e\u52a0\u9176\u91cf\u6216\u8005\u5b75\u80b2\u65f6\u95f4\u3002<\/p>\n\n\n\n

\u53cd\u5e94<\/strong>\u6761\u4ef6<\/strong><\/td>\u6700\u4f73<\/strong><\/td>\u6709\u6548<\/strong><\/td><\/tr>
Mg2<\/strong>+<\/strong><\/td>1-2Mm<\/td>1-10mM<\/td><\/tr>
PH<\/strong><\/td>8.0-9.2<\/td>6.0-10.0<\/td><\/tr>
\u6e29\u5ea6<\/strong><\/td>37\u2103<\/td>0-42\u2103<\/td><\/tr>
DTT<\/strong><\/td>0-100mM<\/td>\u5927\u4e8e100mM<\/td><\/tr>
BME<\/strong><\/td>0-100mM<\/td>\u5927\u4e8e100 mM<\/td><\/tr>
\u94a0\u79bb\u5b50<\/strong>\u3001\u94be\u79bb\u5b50\u7b49<\/strong><\/td>0-20mM<\/td>0-150 mM<\/td><\/tr>
PO<\/strong>4<\/sub><\/strong><\/sub><\/strong>3-<\/sup><\/strong><\/sup><\/strong><\/td>0-10mM<\/td>0-100mM<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u6700\u4f73\u6761\u4ef6\u4e3a\u9176\u6d3b\u4fdd\u755990%\u4ee5\u4e0a\u7684\u53cd\u5e94\u6761\u4ef6\uff1b\u6709\u6548\u6761\u4ef6\u4e3a\u9176\u6d3b\u4fdd\u755915%\u4ee5\u4e0a\u7684\u53cd\u5e94\u6761\u4ef6\u3002<\/p>\n\n\n\n

\u56db. \u50a8\u5b58\u548c\u8fd0\u8f93\u7a33\u5b9a\u6027<\/strong><\/strong><\/p>\n\n\n\n

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\u8fd0\u8f93\u7a33\u5b9a\u6027\uff1a\u51b7\u85cf\u8fd0\u8f93\uff0c\u6d3b\u6027\u7a33\u5b9a\u3002<\/p>\n\n\n\n

\u6ce8\u610f\uff1a\u672c\u4ea7\u54c1\u4e0d\u5efa\u8bae-800<\/sup>C \u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4f1a\u964d\u4f4e\u672c\u4ea7\u54c1\u7684\u9176\u6d3b\u6027\u3002<\/p>\n\n\n\n

\u4e94. \u4ea7\u54c1\u4f18\u52bf<\/strong><\/strong><\/p>\n\n\n\n

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\u7eaf\u5ea6\u9ad8\uff1a\u6bd4\u6d3b\u9ad8\uff1b\u5bbf\u4e3b\u86cb\u767d\u6b8b\u7559\u5c0f\u4e8e\u751f\u7269\u5236\u54c1\u9650\u5ea6\u8981\u6c42\u3002<\/p>\n\n\n\n

\u7b26\u5408\u6cd5\u89c4\u8981\u6c42\uff1a\u751f\u4ea7\u8bbe\u5907\u548c\u751f\u4ea7\u73af\u5883\u7b26\u5408\u76f8\u5173\u6cd5\u89c4\u8981\u6c42\uff0c\u751f\u4ea7\u8fc7\u7a0b\u5b8c\u5168\u7b26\u5408GMP\u6307\u5bfc\u539f\u5219\u3002<\/p>\n\n\n\n

\u8d28\u91cf\u6587\u4ef6\u5b8c\u6574\uff1a\u6309\u5ba2\u6237\u9700\u6c42\uff0c\u53ef\u63d0\u4f9b\u76f8\u5173\u6cd5\u89c4\u652f\u6301\u6587\u4ef6\u3002<\/p>\n\n\n\n

\u516d. \u76f8\u5173\u4ea7\u54c1<\/strong><\/strong><\/p>\n\n\n\n

\u91cd\u7ec4sumo\u9176\uff1b<\/p>\n\n\n\n

\u91cd\u7ec4TEV\u9176\uff1b<\/p>\n\n\n\n

\u91cd\u7ec4\u80a0\u6fc0\u9176\u3002<\/p>\n\n\n\n

\u4ec5\u4f9b\u79d1\u7814\u751f\u4ea7\u4f7f\u7528\uff0c\u4e0d\u80fd\u7528\u4e8e\u4eba\u4f53\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6765\u6e90 \uff1a \u91cd\u7ec4\u6838\u9178\u9176\uff0c\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002 \u4e00. \u4ea7\u54c1\u7b80\u4ecb BenzoNuclease \u662f\u4e00 […]<\/p>\n

Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-681","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/681","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=681"}],"version-history":[{"count":2,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/681\/revisions"}],"predecessor-version":[{"id":736,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/681\/revisions\/736"}],"wp:attachment":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=681"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=681"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=681"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}