{"id":683,"date":"2023-03-15T14:36:39","date_gmt":"2023-03-15T06:36:39","guid":{"rendered":"https:\/\/www.cellnuo.com\/?page_id=683"},"modified":"2024-03-20T10:08:23","modified_gmt":"2024-03-20T02:08:23","slug":"%e9%87%8d%e7%bb%84-cas12b","status":"publish","type":"page","link":"https:\/\/www.cellnuo.com\/?page_id=683","title":{"rendered":"\u91cd\u7ec4 Cas12b"},"content":{"rendered":"\n

\u6765\u6e90 \uff1a<\/strong> \u91cd\u7ec4Cas12b\uff0c\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002<\/p>\n\n\n\n

\u4e00. \u4ea7\u54c1\u7b80\u4ecb<\/strong><\/strong><\/p>\n\n\n\n

Crispr Cas12b\u6838\u9178\u9176\uff08\u53c8\u540dC2c1\uff09\u662f\u4f9d\u8d56\u4e8eRNA\u4ecb\u5bfc\u7684\u5185\u5207\u6838\u9178\u9176\uff0c\u5728\u9776\u6807DNA\u5b58\u5728PAM\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u7279\u5f02\u5730\u5207\u5272\u9776\u6807\u53cc\u94feDNA\u3002Cas12b\u86cb\u767d\u6bd4Cas9\u548cCas12a\u66f4\u5c0f\uff0c\u6765\u6e90\u4e8e\u55dc\u9178\u8010\u70ed\u83cc\u4e14\u5207\u5272\u6d3b\u6027\u8f83\u9ad8\u7684Alicyclobacillus acidoterrestris \u3002Cas12b\u7684\u6700\u4f73\u5207\u5272\u53cd\u5e94\u6e29\u5ea6\u4e3a48 \u2103\uff0c\u548c\u540c\u5bb6\u65cf\u7684Cas12a\u7c7b\u4f3c\uff0cCas12b\u4e5f\u8bc6\u522b5′-TTN\u7684PAM\u5e8f\u5217\uff0c\u5207\u5272DNA\u540e\u4e5f\u4ea7\u751f\u7c98\u6027\u672b\u7aef\uff1b\u7136\u800c\uff0cCas12b\u662f\u53ccRNA\u5bfc\u5411\uff0c\u4f9d\u8d56\u4e8ecrRNA\u548ctracrRNA\uff08\u6216\u8fde\u63a5\u540e\u5f62\u6210\u7684sgRNA\uff09\u3002<\/p>\n\n\n\n

\u6b64\u5916\uff0cCas12b\u4e0d\u4ec5\u53ef\u4ee5\u5e94\u7528\u4e8e\u9776\u6807dsDNA\u7684\u5207\u5272\uff0c\u8fd8\u53ef\u4ee5\u7528\u4e8e\u9776\u6807\u6838\u9178\u7684\u5feb\u901f\u68c0\u6d4b\uff0c\u8be6\u89c1HOLMESv2\u6838\u9178\u5feb\u68c0\u6280\u672f\u3002\u672c\u4ea7\u54c1\u4e2d\uff08\u5305\u62ecCas12b\u9176\uff09\u90fd\u4e0d\u542b\u4efb\u4f55\u9664Cas12b\u5916\u7684\u5176\u5b83\u6838\u9178\u9176\u6d3b\u6027\u3002Cas12b\u662f\u4e00\u79cd\u57fa\u4e8e CRISPR \u7cfb\u7edf\u7684\u6838\u9178\u9176\uff0c\u76f8\u6bd4Cas9\uff0cCas12b\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\u548c\u6548\u7387\uff0c\u53ef\u7528\u4e8e\u5728\u7ec6\u80de\u4e2d\u6572\u9664\u548c\/\u6216\u6572\u5165\u57fa\u56e0\u3002Cas12b\u7cfb\u7edf\u7531\u5f20\u950b\u56e2\u961f\u5f00\u53d1\u548c\u6539\u9020\u3002<\/p>\n\n\n\n

\u4e8c. \u4ea7\u54c1\u7279\u6027<\/strong><\/p>\n\n\n\n

1<\/td>\u6027\u72b6<\/td>\u900f\u660e\u6db2\u4f53<\/td><\/tr>
2<\/td>\u7eaf\u5ea6<\/td>\u226590%\uff08SDS-PAGE\/SEC-HPLC\uff09<\/td><\/tr>
3<\/td>\u5bbf\u4e3b\u86cb\u767d\u8d28\u6b8b\u7559\u91cf<\/td>\uff1c100ng\/mg<\/td><\/tr>
4<\/td>\u7ec6\u83cc\u5185\u6bd2\u7d20<\/td>\uff1c10EU\/mg<\/td><\/tr>
5<\/td>\u5bbf\u4e3bDNA\u6b8b\u7559\u91cf<\/td>\uff1c10ng\/mg<\/td><\/tr>
6<\/td>DNA\u9176\u6b8b\u7559<\/td>\u9634\u6027<\/td><\/tr>
7<\/td>RNA\u9176\u6b8b\u7559<\/td>\u9634\u6027<\/td><\/tr>
8<\/td>\u5916\u5207\u9176\u6d3b\u6027<\/td>\u226580%<\/td><\/tr>
9<\/td>\u6d53\u5ea6<\/td>\u22655mg\uff08ABS\uff09<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u4e09. \u4ea7\u54c1\u5e94\u7528<\/strong><\/strong><\/p>\n\n\n\n

Cas12b\u5728\u5411\u5bfcRNA\u5f15\u5bfc\u4e0b\u8bc6\u522b\u5e76\u5207\u5272\u9776\u6807\u53cc\u94feDNA\u65f6\uff0c\u5176\u201c\u9644\u5c5e\u5207\u5272\u201d\u6d3b\u6027\u88ab\u6fc0\u6d3b\uff0c\u53ef\u9ad8\u6548\u5207\u5272\u4f53\u7cfb\u4e2d\u975e\u7279\u5f02\u5355\u94feDNA(ssDNA)\u3002\u901a\u8fc7\u8bbe\u8ba1\u4e24\u7aef\u6807\u8bb0\u8367\u5149\u57fa\u56e2\u6216\u8005\u5176\u4ed6\u6807\u8bb0\u7269\u7684ssDNA\u63a2\u9488\uff0c\u53ef\u5b9e\u73b0CRISPR\/Cas12b\u5bf9DNA\u6a21\u677f\u7684\u68c0\u6d4b\u548c\u4fe1\u53f7\u653e\u5927\uff0c\u4ece\u800c\u5b9e\u73b0\u5bf9\u9776\u6807\u7684\u68c0\u6d4b\uff0c\u8be5\u7cfb\u7edf\u7075\u654f\u5ea6\u9ad8\uff0c\u7279\u5f02\u6027\u5f3a\u3002<\/p>\n\n\n\n

\u5e38\u7528\u7684\u68c0\u6d4b\u65b9\u6cd5\u5305\u62ec\u5b9e\u65f6\u8367\u5149\u6cd5\u548c\u80f6\u4f53\u91d1\u5c42\u6790\u6cd5\u3002\u76ee\u524d\u8be5\u7cfb\u7edf\u5df2\u7ecf\u5e7f\u6cdb\u5e94\u7528\u4e8e\u5fae\u751f\u7269\u3001\u690d\u7269\u548c\u52a8\u7269\u7684\u57fa\u56e0\u7f16\u8f91\u4e2d\uff0c\u5e76\u5728\u5206\u5b50\u8bca\u65ad\u65b9\u9762\u5177\u6709\u975e\u5e38\u5927\u7684\u5b9e\u9645\u5e94\u7528\u4ef7\u503c\u3002<\/p>\n\n\n\n

\u7528\u5728\u5206\u5b50\u8bca\u65ad\u4e2d\u76f4\u63a5\u68c0\u6d4bDNA\uff0c\u5982\u679c\u68c0\u6d4bRNA\u9700\u8981\u7ed3\u5408\u5c06DNA\u8f6c\u5f55\u6210RNA\u7684\u9176\u3002<\/p>\n\n\n\n

\u56db<\/strong>. \u50a8\u5b58\u548c\u8fd0\u8f93\u7a33\u5b9a\u6027<\/strong><\/strong><\/p>\n\n\n\n

\u50a8\u5b58\u7a33\u5b9a\u6027\uff1a-25\u81f3-15\u2103\u6e29\u5ea6\u4e0b\uff0c\u4e24\u5e74\u6709\u6548\u3002
\u8fd0\u8f93\u7a33\u5b9a\u6027\uff1a\u51b7\u85cf\u8fd0\u8f93\uff0c\u6d3b\u6027\u7a33\u5b9a\u3002
\u6ce8\u610f\uff1a\u672c\u4ea7\u54c1\u4e0d\u5efa\u8bae-80\u2103\u4fdd\u5b58\uff0c\u53cd\u590d\u51bb\u878d\u4f1a\u964d\u4f4e\u672c\u4ea7\u54c1\u7684\u9176\u6d3b\u6027\u3002<\/p>\n\n\n\n

\u4e94. \u4ea7\u54c1\u4f18\u52bf<\/strong>
1.\u65e0\u52a8\u7269\u6e90\u6027\uff1a\u91cd\u7ec4\u751f\u4ea7\uff0c\u65e0\u5916\u6e90\u6027\u7684\u75c5\u6bd2\u6c61\u67d3\uff0c\u751f\u4ea7\u8fc7\u7a0b\u4e0d\u4f7f\u7528\u4efb\u4f55\u52a8\u7269\u6e90\u539f\u6599\u3002
2.\u8d28\u91cf\u7a33\u5b9a\uff1a\u6279\u91cf\u751f\u4ea7\uff08\u7a33\u5b9a\u751f\u4ea7\u4f9b\u5e94100g\uff09\uff0c\u53ef\u4fdd\u8bc1\u7a33\u5b9a\u8fde\u7eed\u7684\u6279\u6b21\u751f\u4ea7\uff1b\u4ea7\u54c1\u6279\u6b21\u95f4\u65e0\u5dee\u5f02\uff0c\u8d28\u91cf\u7a33\u5b9a\u3002
3.\u7eaf\u5ea6\u9ad8\uff1a\u6bd4\u6d3b\u9ad8\uff1b\u5bbf\u4e3b\u86cb\u767d\u6b8b\u7559\u5c0f\u4e8e\u751f\u7269\u5236\u54c1\u9650\u5ea6\u8981\u6c42\u3002
4.\u7b26\u5408\u6cd5\u89c4\u8981\u6c42\uff1a\u751f\u4ea7\u8bbe\u5907\u548c\u751f\u4ea7\u73af\u5883\u7b26\u5408\u76f8\u5173\u6cd5\u89c4\u8981\u6c42\uff0c\u751f\u4ea7\u8fc7\u7a0b\u5b8c\u5168\u7b26\u5408GMP\u6307\u5bfc\u539f\u5219\u3002
5.\u8d28\u91cf\u6587\u4ef6\u5b8c\u6574\uff1a\u6309\u5ba2\u6237\u9700\u6c42\uff0c\u53ef\u63d0\u4f9b\u76f8\u5173\u6cd5\u89c4\u652f\u6301\u6587\u4ef6\u3002<\/p>\n\n\n\n

\u516d. \u76f8\u5173\u4ea7\u54c1<\/strong>
\u91cd\u7ec4sumo\u9176\uff1b
\u91cd\u7ec4TEV\u9176\uff1b
\u91cd\u7ec4\u80a0\u6fc0\u9176\u3002
\u4ec5\u4f9b\u79d1\u7814\u751f\u4ea7\u4f7f\u7528\uff0c\u4e0d\u80fd\u7528\u4e8e\u4eba\u4f53\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6765\u6e90 \uff1a \u91cd\u7ec4Cas12b\uff0c\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002 \u4e00. \u4ea7\u54c1\u7b80\u4ecb Crispr Cas12b […]<\/p>\n

Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"categories":[21],"tags":[],"class_list":["post-683","page","type-page","status-publish","hentry","category-product-cn-zh"],"_links":{"self":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/683","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=683"}],"version-history":[{"count":4,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/683\/revisions"}],"predecessor-version":[{"id":926,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/683\/revisions\/926"}],"wp:attachment":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}