{"id":693,"date":"2023-03-15T14:40:44","date_gmt":"2023-03-15T06:40:44","guid":{"rendered":"https:\/\/www.cellnuo.com\/?page_id=693"},"modified":"2023-04-11T13:23:52","modified_gmt":"2023-04-11T05:23:52","slug":"igf-1-lr3","status":"publish","type":"page","link":"https:\/\/www.cellnuo.com\/?page_id=693","title":{"rendered":"IGF-1 LR3"},"content":{"rendered":"\n<p><strong>\u6765\u6e90 \uff1a<\/strong>&nbsp;\u91cd\u7ec4\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4ea7\u54c1\u7b80\u4ecb<\/strong><strong><\/strong><\/li>\n<\/ul>\n\n\n\n<p>\u957f\u94feLR3\u80f0\u5c9b\u7d20\u6837\u751f\u957f\u56e0\u5b50(LR3 IGF-1),\u5c5e\u4e8e\u80f0\u5c9b\u7d20\u57fa\u56e0\u5bb6\u65cf,\u4e0e\u80f0\u5c9b\u7d20\u524d\u4f53\u86cb\u767d\u5728\u7ed3\u6784\u4e0a\u7c7b\u4f3c\uff0c\u5177\u6709\u66f4\u597d\u7684\u4fc3\u751f\u957f\u4f5c\u7528\uff0c\u80fd\u4fc3\u8fdb\u591a\u6570\u7ec6\u80de\u7684\u5206\u88c2\u3001\u8fc1\u79fb\u548c\u5206\u5316\u3002<\/p>\n\n\n\n<p>\u957f\u94feLR3\u80f0\u5c9b\u7d20\u6837\u751f\u957f\u56e0\u5b50(Long-R3 IGF-I)\u662f\u4e00\u4e2a\u542b\u670983\u4e2a\u6c28\u57fa\u9178\u7684\u4ebaIGF-I\u7684\u7c7b\u4f3c\u7269\u3002\u4e0e\u5b8c\u6574\u7684\u4ebaIGF-I\u76f8\u6bd4\uff0c\u5728N\u7aef\u589e\u52a0\u4e8613\u4e2a\u6c28\u57fa\u9178\u6b8b\u57fa\u3002\u5c24\u4e3a\u91cd\u8981\u7684\u662f\u7b2c3\u4f4d\u6c28\u57fa\u9178\u6b8b\u57fa\u7531\u5929\u7136\u7684Glu\u66ff\u6362\u4e3aArg (\u56e0\u6b64\u79f0\u4e3aR3)\u3002\u4e0e\u4eba\u5929\u7136\u7684IGF-I\u76f8\u6bd4\uff0cLR3 IGF-I\u5927\u5927\u964d\u4f4e\u4e86\u4e0e\u4ebaIGF-I\u7ed3\u5408\u86cb\u767d\u7684\u7ed3\u5408\u80fd\u529b\u3002\u6e38\u79bb\u6d53\u5ea6\u66f4\u9ad8\uff0c\u56e0\u800c\u663e\u8457\u63d0\u9ad8\u751f\u7269\u5b66\u6d3b\u6027\u3002\u5728\u591a \u6570\u4f53\u5916\u8bd5\u9a8c\u4e2d\uff0cLR3 IGF -I\u5728\u6d53\u5ea6\u4e3a0.2-20 ng ml\u7684\u8303\u56f4\u5185\u5c31\u80fd\u5f88\u597d\u53d1\u6325\u751f\u7269\u5b66\u6d3b\u6027\u3002<\/p>\n\n\n\n<p><strong>\u4e8c. \u4ea7\u54c1\u7279\u6027<\/strong><strong><\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>\u6765\u6e90<\/td><td>\u5927\u80a0\u6746\u83cc<\/td><\/tr><tr><td>\u4ea7\u54c1\u5916\u89c2<\/td><td>\u767d\u8272\u3001\u5fae\u9ec4\u8272\u7c89\u672b<\/td><\/tr><tr><td>\u6bd4\u6d3b<\/td><td>\u5927\u9f20L6\u7ec6\u80de\u682a\u68c0\u6d4b\u6d3b\u6027 &gt; 1.0 \u00d7 105&nbsp;U\/mg\uff1b\u4eba\u7684MCF-7\u7ec6\u80de\u682a\u68c0\u6d4b\u6d3b\u6027&gt; 6.7 \u00d7 105&nbsp;U\/mg<\/td><\/tr><tr><td>\u7eaf\u5ea6\uff08\u86cb\u767d\u7535\u6cf3\uff09<\/td><td>SDS-PAGE\u7535\u6cf3\u7eaf\u5ea6\u9ad8\u8fbe98%\u4ee5\u4e0a\uff0cRP-HPLC\u7eaf\u5ea6\u9ad8\u8fbe90%\u4ee5\u4e0a<\/td><\/tr><tr><td>\u5185\u6bd2\u7d20<\/td><td>&lt; 0.01&nbsp;EU\/\u00b5g<\/td><\/tr><tr><td>\u5206\u5b50\u91cf\uff08\u8d28\u8c31\uff09<\/td><td>9112Da<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u4e09. \u50a8\u5b58\u548c\u8fd0\u8f93\u7a33\u5b9a\u6027<\/strong><\/p>\n\n\n\n<p>\u50a8\u5b58\u7a33\u5b9a\u6027\uff1a-15\u2103\u4ee5\u4e0b\u4fdd\u5b58\uff0c\u4e24\u5e74\u6709\u6548\u3002<\/p>\n\n\n\n<p>\u8fd0\u8f93\u7a33\u5b9a\u6027\uff1a\u51b7\u85cf\u8fd0\u8f93\uff0c\u6d3b\u6027\u7a33\u5b9a\u3002<\/p>\n\n\n\n<p><strong>\u56db. \u4ea7\u54c1\u4f18\u52bf<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u65e0\u52a8\u7269\u6e90\u6027\uff1a\u91cd\u7ec4\u751f\u4ea7\uff0c\u65e0\u5916\u6e90\u6027\u7684\u75c5\u6bd2\u6c61\u67d3\uff0c\u751f\u4ea7\u8fc7\u7a0b\u4e0d\u4f7f\u7528\u4efb\u4f55\u52a8\u7269\u6e90\u539f\u6599\u3002<\/p>\n\n\n\n<p>\u8d28\u91cf\u7a33\u5b9a\uff1a\u6279\u91cf\u751f\u4ea7\uff0810+g\u7ea7\u751f\u4ea7\u548c\u4f9b\u5e94\uff09\uff0c\u53ef\u4fdd\u8bc1\u7a33\u5b9a\u8fde\u7eed\u7684\u6279\u6b21\u751f\u4ea7\uff1b\u4ea7\u54c1\u6279\u6b21\u95f4\u65e0\u5dee\u5f02\uff0c\u8d28\u91cf\u7a33\u5b9a\u3002<\/p>\n\n\n\n<p>\u7eaf\u5ea6\u9ad8\uff1a\u6bd4\u6d3b\u9ad8\uff1b\u5185\u6bd2\u7d20\u3001\u5bbf\u4e3b\u86cb\u767d\u6b8b\u7559\u5c0f\u4e8e\u751f\u7269\u5236\u54c1\u9650\u5ea6\u8981\u6c42\u3002<\/p>\n\n\n\n<p>\u51bb\u5e72\u7c89\uff1a\u6613\u4e8e\u50a8\u5b58\u548c\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p>\u7b26\u5408\u6cd5\u89c4\u8981\u6c42\uff1a\u751f\u4ea7\u8bbe\u5907\u548c\u751f\u4ea7\u73af\u5883\u7b26\u5408\u76f8\u5173\u6cd5\u89c4\u8981\u6c42\uff0c\u751f\u4ea7\u8fc7\u7a0b\u5b8c\u5168\u7b26\u5408GMP\u6307\u5bfc\u539f\u5219\u3002<\/p>\n\n\n\n<p>\u8d28\u91cf\u6587\u4ef6\u5b8c\u6574\uff1a\u6309\u5ba2\u6237\u9700\u6c42\uff0c\u53ef\u63d0\u4f9b\u76f8\u5173\u6cd5\u89c4\u652f\u6301\u6587\u4ef6\u3002<\/p>\n\n\n\n<p><strong>\u4e94. \u4ea7\u54c1\u4f7f\u7528\u4e0e\u5efa\u8bae<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u6eb6\u89e3\u65f6\uff1a\u5efa\u8bae\u7528PBS\u6eb6\u89e3\uff0c\u6eb6\u89e3\u6d53\u5ea6\u4e0d\u8d85\u8fc71mg\/ml\uff0c\u73b0\u7528\u73b0\u914d\u3002<\/p>\n\n\n\n<p>\u7ec6\u80de\u57f9\u517b\u63a8\u8350\u4f7f\u7528\u6d53\u5ea6\uff1a5-100\u03bcg\/L.<\/p>\n\n\n\n<p>\u63a8\u8350\u4f7f\u7528\u7ec6\u80de\u7cfb\uff1aHEK293\u3001CHO\u7ec6\u80de\u751f\u4ea7\u6297\u4f53\u548c\u86cb\u767d\uff1b<\/p>\n\n\n\n<p>\u591a\u80fd\u5e72\u7ec6\u80de\u3001\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u3001\u808c\u8089\u5e72\u7ec6\u80de<\/p>\n\n\n\n<p><strong>\u516d. \u76f8\u5173\u4ea7\u54c1<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u91cd\u7ec4\u4eba\u8868\u76ae\u751f\u957f\u56e0\u5b50rEGF\uff1b<\/p>\n\n\n\n<p>\u91cd\u7ec4\u78b1\u6027\u6210\u7ea4\u7ef4\u751f\u957f\u56e0\u5b50bFGF\uff1b<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u6765\u6e90 \uff1a&nbsp;\u91cd\u7ec4\u57fa\u56e0\u5de5\u7a0b\u751f\u4ea7\uff0c\u5927\u80a0\u6746\u83cc\u8868\u8fbe\u3002 \u957f\u94feLR3\u80f0\u5c9b\u7d20\u6837\u751f\u957f\u56e0\u5b50(LR3 IGF-1),\u5c5e\u4e8e\u80f0 [&hellip;]<\/p>\n<p class=\"more-link\"><a href=\"https:\/\/www.cellnuo.com\/?page_id=693\"  class=\"themebutton\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-693","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/693","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=693"}],"version-history":[{"count":2,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/693\/revisions"}],"predecessor-version":[{"id":735,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=\/wp\/v2\/pages\/693\/revisions\/735"}],"wp:attachment":[{"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=693"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=693"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cellnuo.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}