{"id":839,"date":"2024-02-18T19:25:14","date_gmt":"2024-02-18T11:25:14","guid":{"rendered":"https:\/\/www.cellnuo.com\/?page_id=839"},"modified":"2024-02-18T19:25:15","modified_gmt":"2024-02-18T11:25:15","slug":"recombinant-type-iii-collagen","status":"publish","type":"page","link":"https:\/\/www.cellnuo.com\/?page_id=839&lang=en","title":{"rendered":"Recombinant Type III Collagen"},"content":{"rendered":"\n

Storage: <\/strong>-20\u00b0C or below, protected from light.<\/p>\n\n\n\n

Source: <\/strong>Genetic engineering of pichia pastoris.<\/p>\n\n\n\n

I. Introduction <\/strong><\/strong><\/p>\n\n\n\n

Type III collagen is a main collagen in human skin, fascia and tendons, with a ratio of 1:4 to the type I collagen. <\/a><\/a>It is relatively smaller and exists between the epidermis and dermis, called as \u201cBaby Collagen\u201d. Cushion network, with a main component of the type III collagen, refers to the tissue structure between the epidermis and the dermis, which is the key to supporting the epidermis, and also the first site with the occurrence of skin collapse.<\/p>\n\n\n\n

The purification through heat treatment is applied in this Recombinant Type III Collagen, to express the recombinant pichia pastoris fermentation broth in the recombinant type III collagen. By virtue of this method, the degradation of the recombinant human type III collagen in the fermentation broth into disordered low-molecular-weight peptides can be effectively prevented, and the production of endotoxins in the fermentation broth can also be reduced, thus to greatly improve the purity of the recombinant human type III collagen, to enable it to be a highly purified collagen that meets the specifications of raw material of cosmetics, medical devices and medical-grade products.<\/p>\n\n\n\n

II. Characteristics <\/strong><\/strong><\/p>\n\n\n\n

Item<\/td>Specification<\/td>Result<\/td><\/tr>
Appearance<\/td>White to off-white powder<\/td>Complies<\/td><\/tr>
Purity (by HPLC)<\/td>\u226592%<\/td>95.3%<\/td><\/tr>
Bacterial endotoxin<\/td>\u226410 EU\/mg<\/td>0.5 eu\/mg<\/td><\/tr>
Residual exogenous DNA<\/td>\u226410 ng\/mg<\/td><1 ng\/mg<\/td><\/tr>
Molecular weight<\/td>6,215 Da<\/td>SDS-PAGE<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

III. Storage and Transportation Stability <\/strong><\/strong><\/p>\n\n\n\n

Routine storage at \u2264-20\u00b0C, with no loss of activity for 1 year.<\/p>\n\n\n\n

There is basically no loss of activity for 1 month after thawing under the sterile environment at 2\u20138\u00b0C.<\/p>\n\n\n\n

IV. Advantages <\/strong><\/strong><\/p>\n\n\n\n

Non-animal-derived product: Recombinant production with no contamination of exogenous viruses or use of animal-derived raw materials.<\/p>\n\n\n\n

Stable quality: Mass production (kilogram-scale production and supply) to ensure stable and continuous batch production, with stable quality and no inter-lot difference.<\/p>\n\n\n\n

High purity: The specification of the endotoxin is controlled to be 0.1 EU\/mg, which meets the requirements of injection use.<\/p>\n\n\n\n

Lyophilized powder: Convenient for the storage and transportation.<\/p>\n\n\n\n

Regulatory compliance: The manufacturing equipment and environment conform to relevant regulations, and the manufacturing process fully complies with the GMP Guidelines.<\/p>\n\n\n\n

<\/a>V. Use and Recommendations <\/strong><\/strong><\/p>\n\n\n\n

Dissolution: An acidic solution is recommended for dissolution, and alcohols can be added as a protective agent. Use immediately after dissolution.<\/p>\n\n\n\n

Sterilizing filtration is recommended before use for safety.<\/p>\n\n\n\n

Recommended application: repair masks, devices<\/p>\n\n\n\n

Culture of Vero cells, pluripotent stem cells, mesenchymal stem cells and muscle stem cells.<\/p>\n\n\n\n

VI. Related Products <\/strong><\/strong><\/p>\n\n\n\n

Recombinant Collagen Peptide, GHK-Cu, etc.<\/p>\n","protected":false},"excerpt":{"rendered":"

Storage: -20\u00b0C or below, protected from light. Source: […]<\/p>\n

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