{"id":841,"date":"2024-02-18T19:27:10","date_gmt":"2024-02-18T11:27:10","guid":{"rendered":"https:\/\/www.cellnuo.com\/?page_id=841"},"modified":"2024-02-18T19:27:11","modified_gmt":"2024-02-18T11:27:11","slug":"recombinant-bovine-insulin-rbi","status":"publish","type":"page","link":"https:\/\/www.cellnuo.com\/?page_id=841&lang=en","title":{"rendered":"Recombinant Bovine Insulin (RBI)"},"content":{"rendered":"\n

Storage: <\/strong>-20\u00b0C or below, protected from light<\/p>\n\n\n\n

Source: <\/strong>E. coli<\/em> (BL21)<\/p>\n\n\n\n

I. Introduction <\/strong><\/strong><\/p>\n\n\n\n

Insulin is the only hormone that lowers blood glucose levels in the body and is also a crucial synthetic hormone in living organisms, which can promote the synthesis of glycogens, fats and proteins. Recombinant bovine insulin (rBI) is obtained by biofermentation and purification of Escherichia coli<\/em> (E. coli<\/em>) expression system constructed by genetic engineering technology, with amino acid sequences consistent with those of bovine insulin. rBI is a highly purified, non-animal-derived active protein.<\/p>\n\n\n\n

At present, rBI for industrial use is mainly utilized as an additive to animal cell culture media. Cell culture media supplemented with rBI can better mimic the in vivo<\/em> growth and metabolic environment, which helps to accelerate cell growth, increase cell density, and promote stable expression of target proteins (viral titers).<\/p>\n\n\n\n

II. Characteristics <\/strong><\/strong><\/p>\n\n\n\n

Item <\/strong><\/strong><\/td>Specification <\/strong><\/strong><\/td>Method <\/strong><\/strong><\/td><\/tr>
Appearance<\/td>White or off-white crystalline powder<\/td>Visual inspection<\/td><\/tr>
Purity<\/td>\u226590%<\/td>SDS-PAGE<\/td><\/tr>
Bacterial endotoxin<\/td>\u226410 EU\/mg<\/td>Test method in Pharmacopeia<\/td><\/tr>
Host DNA<\/td>\u226410 ppm<\/td>Fluorescent PCR<\/td><\/tr>
Residual host cell protein (HCP)<\/td>\u226450 ppm<\/td>Test method in Pharmacopeia<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

III. Storage and Transportation Stability<\/strong><\/p>\n\n\n\n

Preserve in tight containers, protected from light. Lyophilized powder is stable for 24 months when stored below -15\u00b0C.<\/p>\n\n\n\n

If not used immediately, store temporarily at 2-8\u00b0C, protected from light.<\/p>\n\n\n\n

Transportation stability: This product is stable during insulated transportation with ice bags or dry ice.<\/p>\n\n\n\n

IV. Advantages <\/strong><\/strong><\/p>\n\n\n\n

This product has the advantages of high purity, high activity, non-animal origin, and low levels of residual host cell protein and DNA.<\/p>\n\n\n\n

This product is produced and supplied at the kilogram scale. Lyophilized powder is easy for transportation and storage.<\/p>\n\n\n\n

This product has a mature and stable manufacturing process and also has good inter-lot stability. Regulatory compliance: The manufacturing equipment and environment comply with relevant regulations. The manufacturing process complies with GMP guidelines.<\/p>\n\n\n\n

V. Use and Recommendations <\/strong><\/strong><\/p>\n\n\n\n

Dissolution: Directly dissolve in 10 mM PBS. Prepare freshly before use.<\/p>\n\n\n\n

Storage after dissolution: Adjust pH to 8.0 after dissolution, and if not used immediately, store temporarily at 2-8\u00b0C. It is not recommended to store under acidic conditions. Repeated freezing and thawing is not allowed.<\/p>\n\n\n\n

Recommended concentration for cell culture: 2-20 mg\/L.<\/p>\n\n\n\n

Recommended cell lines: Bovine pluripotent stem cells, mesenchymal stem cells, and muscle stem cells;<\/p>\n\n\n\n

CHO cells and HEK293 cells for production of antibodies and proteins;<\/p>\n\n\n\n

Vero cells, BHK cells and diploid cells for production of virus particles.<\/p>\n","protected":false},"excerpt":{"rendered":"

Storage: -20\u00b0C or below, protected from light Source: E […]<\/p>\n

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